Figure 1: Description and proof of principle for balanced PCR. As an alternative to the separation PCR step, LN1- and LN2-ligated plasmid DNA were mixed in different proportions and used in a ...

A 1.4-Mb tandem duplication, including the gene for peripheral myelin protein 22 (PMP22) in chromosome 17p11.2-12 is responsible for 70% of the cases of the demyelinating type 1 of Charcot-Marie-Tooth ...

The evolution of PCR technology is shaping the future of diagnostics, especially in viral detection and RNA analysis. In the second episode of our New Frontiers in PCR Technology Series, we have ...

Polymerase Chain Reaction (PCR) is a method which utilizes the mechanism of DNA replication to amplify a specific part of DNA. This involves the use of DNA polymerase, which makes new strands of DNA ...

In the six decades since Watson and Crick identified the double helical structure of DNA, a series of elegant and powerful experiments has established the broad outlines of how genetic information is ...

Quantitative polymerase chain reaction, or "qPCR", is considered the “second generation” of PCR and was first described in 1992. The past three decades have seen significant advances in qPCR ...

Quantitative PCR (qPCR) is a sensitive molecular technique used to measure the expression levels of specific genes across different samples. In our research, we use qPCR to quantify transcriptional ...

There are many different technologies available to conduct multiplex PCR. However, each comes with its own sets of pros and cons. This infographic compares three pillars of PCR, quantitative PCR, ...